文章基本信息

标题：Metabolic Profiling-Based Evaluation of the Fermentative Behavior of Aspergillus oryzae and Bacillus subtilis for Soybean Residues Treated at Different Temperatures
本地全文：下载
作者：Hyejin Hyeon ; Cheol Woo Min ; Keumok Moon 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：2
页码：117-133
DOI：10.3390/foods9020117
出版社：MDPI Publishing
摘要：Soybean processing, e.g., by soaking, heating, and fermentation, typically results in diverse metabolic changes. Herein, multivariate analysis-based metabolic profiling was employed to investigate the effects of fermentation by Aspergillus oryzae or Bacillus subtilis on soybean substrates extracted at 4, 25, or 55 °C. As metabolic changes for both A. oryzae and B. subtilis were most pronounced for substrates extracted at 55 °C, this temperature was selected to compare the two microbial fermentation strategies, which were shown to be markedly different. Specifically, fermentation by A. oryzae increased the levels of most organic acids, γ-aminobutyric acid, and glutamine, which were ascribed to carbohydrate metabolism and conversion of glutamic acid into GABA and glutamine. In contrast, fermentation by B. subtilis increased the levels of most amino acids and isoflavones, which indicated the high activity of proteases and β-glucosidase. Overall, the obtained results were concluded to be useful for the optimization of processing steps in terms of nutritional preferences.
关键词：soybean substrate; fermentation; Aspergillus oryzae ; Bacillus subtilis ; extraction temperature; metabolic profiling; multivariate analysis soybean substrate ; fermentation ; Aspergillus oryzae ; Bacillus subtilis ; extraction temperature ; metabolic profiling ; multivariate analysis