文章基本信息

标题：Production of Bio-Functional Protein through Revalorization of Apricot Kernel Cake
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作者：Jelena Čakarević ; Senka Vidović ; Jelena Vladić 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2019
卷号：8
期号：8
页码：318-329
DOI：10.3390/foods8080318
出版社：MDPI Publishing
摘要：The study describes and compares bio-functional properties and thein vitrodigestibility of protein isolates from apricot oil cakes obtained by supercritical fluid extraction and cold pressing, as control. Protein isolates have the potential to be food ingredients with amygdalin contents in an amount considerably lower than regulatory. Isolates showed hypoglycemic activity, studied by the inhibition of α-glucosidase, also functional properties were determined.Good digestibility of proteins, which were done using gastrointestinal proteases (pepsin and pancreatin) were proven by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis SDS-PAGE analysis. Moreover, it was evident that the protein isolates were completely digested. The biologically active potential of the digests was evaluated measuring in vitro antioxidant capacity by three complementary methods and enzyme inhibitory effects towards Angiotensin-I Converting Enzyme (ACE) related with the onset of hypertension. All hydrolysates act as a DPPH and ABTS scavenger, as a reducing agents and an ACE enzyme inhibitor. In conclusion, protein isolates obtained from apricot kernel cake showed to be a promising source of natural products for food applications, with good functional and bioactive properties and easy digestibility.
关键词：kernel protein; supercritical CO 2 extraction; in vitro digestion; Angiotensin-I Converting Enzyme (ACE) inhibition; antioxidant activity kernel protein ; supercritical CO 2 extraction ; in vitro digestion ; Angiotensin-I Converting Enzyme (ACE) inhibition ; antioxidant activity