文章基本信息

标题：A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain
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作者：Giulia Cibecchini ; Paola Cecere ; Giorgio Tumino 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：11
页码：1691-1700
DOI：10.3390/foods9111691
出版社：MDPI Publishing
摘要：The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for easy and clear identification of specific wheat varieties was developed. Notably, an approach based on the loop-mediated isothermal amplification reaction (LAMP) as an SNP discrimination tool, in combination with naked-eye visualization of the results, was designed and optimized. Our assay was proven to be effective in the detection of adulterated food products, including both substitution and mixing with different crop varieties.
关键词：durum wheat variety; genetic traceability; single nucleotide polymorphisms; colorimetric tests; authenticity; point of care test durum wheat variety ; genetic traceability ; single nucleotide polymorphisms ; colorimetric tests ; authenticity ; point of care test