文章基本信息

标题：Changes in Protein Non-Covalent Bonds and Aggregate Size during Dough Formation
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作者：Sonoo Iwaki ; Shiro Aono ; Katsuyuki Hayakawa 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：11
页码：1643-1656
DOI：10.3390/foods9111643
出版社：MDPI Publishing
摘要：This research investigated changes in the amounts and sizes of monomeric proteins and protein aggregates during dough mixing, with a focus on the contribution of non-covalent bonds in the aggregation of gluten proteins. High protein flour (HF) and low protein flour (LF) were used in this study. As dough mixing progressed from flour to overmixed dough, the total amount of protein aggregates increased while the amount of monomeric protein decreased. Omega-gliadin was the major monomeric protein that decreased in quantity. Interestingly, the amount of larger-sized protein aggregates decreased and that of smaller-sized protein aggregates increased. The amount of gluten protein macro-polymer aggregated through strong non-covalent bonds decreased whereas aggregates formed with weaker non-covalent bonds increased. LF dough behaved similar to HF dough. Large-sized gluten protein aggregates disaggregated due to the weakening of non-covalent bonds and became smaller. Omega-gliadin was incorporated into gluten protein aggregates during dough mixing.
关键词：wheat; flour; dough; mixing; non-covalent bond; aggregation wheat ; flour ; dough ; mixing ; non-covalent bond ; aggregation