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标题：Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry
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作者：Gilda Aiello ; Yuchen Li ; Giovanna Boschin 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：8
页码：1083-1095
DOI：10.3390/foods9081083
出版社：MDPI Publishing
摘要：The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches based on liquid chromatography−electrospray ionization−tandem mass spectrometry (LC−ESI−MS/MS) for investigating the protein profile of three pasta samples containing different percentages of narrow-leaf lupin flour were carried out. The untargeted method permitted the identification of the main acidic globulins (α-conglutin, β-conglutin, and δ-conglutin) and the comparison of their profile with raw lupin flour. The targeted method, based on High-performance liquid chromatography electrospray ionization tandem mass spectrometry HPLC-Chip-Multiple Reaction Monitoring (MRM) mode, allowed the quantification of γ-conglutin, the main hypoglycemic component of lupin protein: its concentration was around 2.25 mg/g in sample A, 2.16 mg/g in sample D, and 0.57 mg/g in sample F.
关键词：food quality; liquid chromatography-tandem mass spectrometry LC-MS/MS; functional foods; lupin-enriched pasta; label free quantification; γ-conglutin; Lupinus angustifolius food quality ; liquid chromatography-tandem mass spectrometry LC-MS/MS ; functional foods ; lupin-enriched pasta ; label free quantification ; γ-conglutin ; Lupinus angustifolius