文章基本信息

标题：High Hydrostatic Pressure Assisted by Celluclast® Releases Oligosaccharides from Apple By-Product
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作者：Rocío De la Peña-Armada ; María José Villanueva-Suárez ; Pilar Rupérez 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：8
页码：1058-1070
DOI：10.3390/foods9081058
出版社：MDPI Publishing
摘要：A novel and green procedure consisting of high hydrostatic pressure (HHP) aided by a commercial cellulase (Celluclast®) has been applied to valorise the apple by-product, a valuable source of dietary fibre but mainly composed by insoluble fibre. Optimal conditions for solubilisation of dietary fibre were first determined at atmospheric pressure as 2% (w/v) of substrate concentration and 20 Endo-Glucanase Units of cellulase. Monitoring of polysaccharides and oligosaccharides released from apple by-product was carried out by means of a newly validated HPLC method with refractive index detector. A synergistic effect was observed when the combined HHP plus cellulase treatment was used. Thus, the application of 200 MPa at 50 °C for 15 min enabled a significant increase in the release of water-soluble polysaccharides (1.8-fold) and oligosaccharides (3.8-fold), as well as a considerable decrease in the time required (up to 120-fold), compared to control at 0.1 MPa. Therefore, this technology could be a promising alternative approach to transform an industrial by-product into a novel rich-in-oligosaccharide food ingredient and a step forward into shaping the world of prebiotics.
关键词：apple by-product; valorisation; green technology; HHP; cellulase; dietary fibre; polysaccharides; oligosaccharides; prebiotics apple by-product ; valorisation ; green technology ; HHP ; cellulase ; dietary fibre ; polysaccharides ; oligosaccharides ; prebiotics