文章基本信息

标题：Modeling of Multivalent Ligand-Receptor Binding Measured by kinITC
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作者：Franziska Erlekam ; Sinaida Igde ; Susanna Röblitz 等
期刊名称：Computation
电子版ISSN：2079-3197
出版年度：2019
卷号：7
期号：3
页码：46-61
DOI：10.3390/computation7030046
出版社：MDPI Publishing
摘要：In addition to the conventional Isothermal Titration Calorimetry (ITC), kinetic ITC (kinITC) not only gains thermodynamic information, but also kinetic data from a biochemical binding process. Moreover, kinITC gives insights into reactions consisting of two separate kinetic steps, such as protein folding or sequential binding processes. The ITC method alone cannot deliver kinetic parameters, especially not for multivalent bindings. This paper describes how to solve the problem using kinITC and an invariant subspace projection. The algorithm is tested for multivalent systems with different valencies.
关键词：multivalent bindings; PCCA ; kinetic ITC multivalent bindings ; PCCA ; kinetic ITC