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标题：Disturbing-Free Determination of Yeast Concentration in DI Water and in Glucose Using Impedance Biochips
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作者：Mahdi Kiani ; Nan Du ; Manja Vogel 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：1
页码：7-23
DOI：10.3390/bios10010007
出版社：MDPI Publishing
摘要：Deionized water and glucose without yeast and with yeast (Saccharomyces cerevisiae) of optical density OD600 that ranges from 4 to 16 has been put in the ring electrode region of six different types of impedance biochips and impedance has been measured in dependence on the added volume (20, 21, 22, 23, 24, 25 µL). The measured impedance of two out of the six types of biochips is strongly sensitive to the addition of both liquid without yeast and liquid with yeast and modelled impedance reveals a linear relationship between the impedance model parameters and yeast concentration. The presented biochips allow for continuous impedance measurements without interrupting the cultivation of the yeast. A multiparameter fit of the impedance model parameters allows for determining the concentration of yeast (cy) in the range from cy = 3.3 × 107 to cy = 17 × 107 cells/mL. This work shows that independent on the liquid, i.e., DI water or glucose, the impedance model parameters of the two most sensitive types of biochips with liquid without yeast and with liquid with yeast are clearly distinguishable for the two most sensitive types of biochips.
关键词：biochips; impedance spectroscopy; yeast Saccharomyces cerevisiae ; electrical equivalent circuit; biomaterial; biosensing biochips ; impedance spectroscopy ; yeast Saccharomyces cerevisiae ; electrical equivalent circuit ; biomaterial ; biosensing