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标题：Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode
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作者：Myungsang Park ; Yesol Song ; Ki Jun Kim 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：4
页码：38-49
DOI：10.3390/bios10040038
出版社：MDPI Publishing
摘要：The immunoglobulin E (IgE) level in serum is an important factor in the examination of allergy. Ferrocene (Fc)-modified self-assembled monolayers (SAMs) were placed on an indium tin oxide (ITO) electrode as a sensing layer for the detection of human IgE. The Fc moiety in the SAMs facilitated the electron transfer through the organic SAMs layer and electrocatalytic signal amplification. The electrochemical measurement was accomplished after the sandwich type immobilization of the receptor antibody, target human IgE, and enzyme conjugated secondary antibody. The enzyme product, p-aminophenol, was quantitatively analyzed by redox cycling via Fc. In addition, the electrochemical impedance spectroscopy (EIS) was investigated for the detection of IgE. The limit of detection (LOD), limit of quantification (LOQ), and dynamic range of the electrochemical sensor were 3 IU/mL, 10 IU/mL, and from 10 IU/mL to 100 IU/mL, respectively.
关键词：human immunoglobulin E; indium tin oxide (ITO); electrocatalytic reaction; ferrocene; electrochemical impedance spectroscopy human immunoglobulin E ; indium tin oxide (ITO) ; electrocatalytic reaction ; ferrocene ; electrochemical impedance spectroscopy