文章基本信息

标题：Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
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作者：Sandro Spagnolo ; Brian De La Franier ; Tibor Hianik 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：3
页码：20-28
DOI：10.3390/bios10030020
出版社：MDPI Publishing
摘要：This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties.
关键词：anti-fouling monolayer; electromagnetic piezoelectric acoustic sensor; blood serum; milk anti-fouling monolayer ; electromagnetic piezoelectric acoustic sensor ; blood serum ; milk