文章基本信息

标题：Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach
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作者：Lisa Crossley ; Bukola Attoye ; Vincent Vezza 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2019
卷号：9
期号：4
页码：141-152
DOI：10.3390/bios9040141
出版社：MDPI Publishing
摘要：We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences.
关键词：field-effect transistor; biosensor; TP53; electrochemistry; open-circuit potential field-effect transistor ; biosensor ; TP53 ; electrochemistry ; open-circuit potential