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标题：Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques
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作者：Carmen M. González-Henríquez ; Vanessa A. Villegas-Opazo ; Dallits H. Sagredo-Oyarce 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2017
卷号：7
期号：3
页码：34-50
DOI：10.3390/bios7030034
出版社：MDPI Publishing
摘要：Biomimetic planar artificial membranes have been widely studied due to their multiple applications in several research fields. Their humectation and thermal response are crucial for reaching stability; these characteristics are related to the molecular organization inside the bilayer, which is affected by the aliphatic chain length, saturations, and molecule polarity, among others. Bilayer stability becomes a fundamental factor when technological devices are developed—like biosensors—based on those systems. Thermal studies were performed for different types of phosphatidylcholine (PC) molecules: two pure PC bilayers and four binary PC mixtures. These analyses were carried out through the detection of slight changes in their optical and structural parameters via Ellipsometry and Surface Plasmon Resonance (SPR) techniques. Phospholipid bilayers were prepared by Langmuir-Blodgett technique and deposited over a hydrophilic silicon wafer. Their molecular inclination degree, mobility, and stability of the different phases were detected and analyzed through bilayer thickness changes and their optical phase-amplitude response. Results show that certain binary lipid mixtures—with differences in its aliphatic chain length—present a co-existence of two thermal responses due to non-ideal mixing.
关键词：binary phospholipid mixture; heterogeneous membranes; thermal phase transitions; ellipsometry; surface plasmon resonance binary phospholipid mixture ; heterogeneous membranes ; thermal phase transitions ; ellipsometry ; surface plasmon resonance