文章基本信息

标题：A Liposomal Platform for Sensing of Extracellular Analytes Near Cells
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作者：Xiaozhou Zhang ; Sabrina Heng ; Jinxin Pei 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2018
卷号：8
期号：4
页码：117-127
DOI：10.3390/bios8040117
出版社：MDPI Publishing
摘要：Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2 , GSH, and Zn2 , respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2 (0.25 mM–1 mM), Zn2 (6.25 µM–50 µM), and GSH (0.25 mM–1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2 (1 mM), Ca2 (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications.
关键词：liposome; biosensing; extracellular sensing; fluorescence; small-molecule sensors liposome ; biosensing ; extracellular sensing ; fluorescence ; small-molecule sensors