文章基本信息

标题：Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis
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作者：Cristina Gordillo-Marroquín ; Anaximandro Gómez-Velasco ; Héctor J. Sánchez-Pérez 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2018
卷号：8
期号：4
页码：128-140
DOI：10.3390/bios8040128
出版社：MDPI Publishing
摘要：A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1 ” (in SSM) to “2 ”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1 ”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs.
关键词：Mycobacterium tuberculosis ; increased sensitivity; sputum smear microscopy; TB detection; nanotechnology; infectious disease Mycobacterium tuberculosis ; increased sensitivity ; sputum smear microscopy ; TB detection ; nanotechnology ; infectious disease