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标题：Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells
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作者：Thanih Balbaied ; Anna Hogan ; Eric Moore 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：8
页码：95-108
DOI：10.3390/bios10080095
出版社：MDPI Publishing
摘要：Alkaline phosphatase (ALP) is one of the main biomarkers that is clinically detected in bone and liver disorders using optical assays. The electrochemical principle is important because point-of-care testing is increasing dramatically and absorbance techniques hardly compete with the medical revolution that is occurring. The detection of ALP using electrochemical detection is contributing to the integration systems field, and hence enhancing the detection of biological targets for pharmaceutical research and design systems. Moreover, in vitro electrochemical measurements use cost effective materials and simple techniques. Graphite screen-printed electrodes and linear sweep voltammetry were used to optimize the electrochemistry of the enzymatic product p-aminophenol using the enzyme kinetic assay. ALP release from embryonic and cancer cells was determined from adhesion cell culture. Additionally, capillary electrophoresis and colorimetric methods were applied for comparison assays. The resulting assays showed a dynamic range of ALP ranging from 1.5 to 1500 U/L, and limit of detection of 0.043 U/L. This was achieved by using 70 μL of the sample and an incubation time of 10 min at an optimal substrate concentration of 9.6 mM of p-aminophenol phosphate. A significant difference (p < 0.05) was measured between the absorbance assays. This paper demonstrates the advantages of the electrochemical assay for ALP release from cells, which is in line with recent trends in gene expression systems using microelectrode array technologies and devices for monitoring electrophysiological activity.
关键词：electrochemistry; linear sweep voltammetry; alkaline phosphatase; capillary electrophoresis; colorimetry; cancer cells; embryonic cells electrochemistry ; linear sweep voltammetry ; alkaline phosphatase ; capillary electrophoresis ; colorimetry ; cancer cells ; embryonic cells