文章基本信息

标题：Expanded newborn screening program in Slovenia using tandem mass spectrometry and confirmatory next generation sequencing genetic testing
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作者：Barbka Repič Lampret ; Žiga Iztok Remec ; Ana Drole Torkar 等
期刊名称：Slovenian Journal of Public Health
印刷版ISSN：0351-0026
电子版ISSN：1854-2476
出版年度：2020
卷号：59
期号：4
页码：256-263
DOI：10.2478/sjph-2020-0032
出版社：Walter de Gruyter GmbH
摘要：
Introduction
In the last two decades, the introduction of tandem mass spectrometry in clinical laboratories has enabled simultaneous testing of numerous acylcarnitines and amino acids from dried blood spots for detecting many aminoacidopathies, organic acidurias and fatty acid oxidation disorders. The expanded newborn screening was introduced in Slovenia in September 2018. Seventeen metabolic diseases have been added to the pre-existing screening panel for congenital hypothyroidism and phenylketonuria, and the newborn screening program was substantially reorganized and upgraded.
Methods
Tandem mass spectrometry was used for the screening of dried blood spot samples. Next-generation sequencing was introduced for confirmatory testing. Existing heterogeneous hospital information systems were connected to the same laboratory information system to allow barcode identification of samples, creating reports, and providing information necessary for interpreting the results.
Results
In t he first y ear of t he expanded newborn screening a total of 15,064 samples w ere screened. Four patients were confirmed positive with additional testing.
Conclusions
An expanded newborn screening program was successfully implemented with the first patients diagnosed before severe clinical consequences.
关键词：newborn screening ; NBS ; tandem mass spectrometry ; MS/ MS ; next-generation sequencing ; NGS ; inborn errors of metabolism ; IEM

文章基本信息

Introduction

Methods

Results

Conclusions