文章基本信息

标题：Effect of miR-215 on the Expression of Tumor Suppressor Gene Rb1 in Retinoblastoma Cell Lines
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作者：Liqin SHAO ; Zhangxing SHENG ; Yuefeng ZHU 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2020
卷号：49
期号：7
页码：1298-1306
DOI：10.18502/ijph.v49i7.3583
出版社：THE SCHOOL OF PUBLIC HEALTH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES
摘要：Background: Effect of miR-215 on the expression of tumor suppressor gene retinoblastoma (Rb)1 in Rb cell lines was investigated. Methods: A total of 128 patients were selected. The expression of miR-215 in cancer and adjacent healthy tissues of the 128 patients was detected by reverse transcription-quantitative PCR (RT-qPCR). HXO-Rb44 and Y79 cell lines were transfected with miR-215 analogs or miR-215 inhibitors, and the expression of Rb1 protein in the cell lines was detected by western blotting. Results: The expression of miR-215 in the adjacent healthy tissues of patients was significantly lower than that in cancer tissues ( P< 0.001). The expression of miR-215 in Y79 and HXO-Rb44 cells was significantly higher than that in APRE-19 cells ( P< 0.001). The expression of miR-215 in HXO-Rb44 cells was significantly higher than that in Y79 cells ( P< 0.001). The expression of miR-215 was statistically different from the degree of differentiation and nerve infiltration ( P< 0.05). The expression of Rb1 in cancer tissues was significantly lower than that in adjacent tissues ( P< 0.001), the expression of APRE-19 was significantly higher than that in Y79 and HXO-Rb44 cells ( P< 0.001), and the expression of Rb1 in HXO-Rb44 cells was significantly higher than that in Y79 cells ( P< 0.05). There was a negative correlation between miR-215 and Rb1 in the tissues of patients, and Rb1 expression decreased with the increase of miR-215 (r=-0.576, P< 0.001). Conclusion: miR-215 is highly expressed in Rb cell lines, and is related to the clinicopathological features of this disease.
关键词：miR-215; Retinoblastoma; Retinoblastoma (Rb)