文章基本信息

标题：Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from the pig production chain in Northern Italy
本地全文：下载
作者：Ancuta Cezara Simon ; Valentina Baldo ; Nadia Losio 等
期刊名称：Italian Journal of Food Safety
电子版ISSN：2239-7132
出版年度：2020
卷号：9
期号：2
DOI：10.4081/ijfs.2020.8412
出版社：PAGEPress Publications
摘要：This study aimed to evaluate the molecular characteristics of methicillin resistant Staphylococcus aureus (MRSA) isolated in the swine chain in Northern Italy. A sample of 50 fattening units located in Lombardy was selected. Five cutaneous samples at slaughtering and three environmental samples at farm were collected from each unit giving a total of 250 and 150 samples, respectively. A total of 25 MRSA isolates were isolated from 400 samples, in 17 different fattening units. At farm, 12 out of 250 samples were positive for MRSA (4,8 %), and 13 out of 150 samples at slaughter were identified as MRSA (8,7 %), giving an overall incidence among samples of 6,25 % (n = 25). Molecular characterization was carried out using multi-locus sequence typing (MLST) and spa-typing. Outcomes showed that most of the isolates belonged to ST398, carrying spa-types t899, t011, t18498, t1939, t1200, and t304. Nonetheless, three isolates were identified as ST97 (t1730 and t4795), and one as ST30, showing spa-type t318. Furthermore, a novel ST was identified, namely 5422, showing spa-type t1730. Heterogeneity in genotypes within the same farm was also found in different fattening units, with concern for the possibility of the exchange of genetic determinants among different lineages. Genetic diversity among MRSA isolates in pig fattening units has been observed, highlighting the possibility that some isolates could be able to infect different hosts, including human.
关键词：MRSA;MLST;Spa-typing;Pigs;Swine chain;Northern Italy