文章基本信息

标题：Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes
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作者：Stuart Hannah ; Alexandra Dobrea ; Perrine Lasserre 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：11
页码：153
DOI：10.3390/bios10110153
出版社：MDPI Publishing
摘要：Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, Escherichia coli (E. coli) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the E. coli growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (p < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of E. coli within clinically relevant concentration ranges for conditions such as urinary tract infections.
关键词：antibiotic susceptibility testing (AST); Escherichia coli ( E. coli) ; electrochemistry; screen-printed electrodes (SPEs); streptomycin; growth-profiles; real-time monitoring antibiotic susceptibility testing (AST) ; Escherichia coli ( E. coli) ; electrochemistry ; screen-printed electrodes (SPEs) ; streptomycin ; growth-profiles ; real-time monitoring