文章基本信息

标题：60 specific eDNA qPCR assays to detect invasive, threatened, and exploited freshwater vertebrates and invertebrates in Eastern Canada
本地全文：下载
作者：Cecilia Hernandez ; Bérénice Bougas ; Alysse Perreault‐Payette 等
期刊名称：Environmental DNA
电子版ISSN：2637-4943
出版年度：2020
卷号：2
期号：3
页码：373-386
DOI：10.1002/edn3.89
出版社：Wiley
摘要：Practical applications of environmental DNA (eDNA) are in exponential expansion, especially for the assessment and monitoring of freshwater metazoans. Because eDNA sampling and analysis is noninvasive, it improves the detection of threatened, invasive, and exploited species for which monitoring may be challenging. Species detection efforts using a combination of eDNA and qPCR have been highly successful and, as a result, their use in species monitoring is expanding rapidly. We developed qPCR primers and probes in order to monitor many invasive, threatened, or exploited aquatic species as part of various monitoring eDNA projects in the province of Québec, Canada. Here, we present a total of 60 species‐specific qPCR assays (including PCR protocols, primers, and TaqMan probes sequences) developed for the detection of 45 fishes, six amphibians, five reptiles, two mollusks, and two crustaceans. These comprised nine and 27 species, respectively, listed as invasive and threatened in Eastern Canada. These resources should be of broad usefulness not only for monitoring studies based in Québec but throughout the geographic range of the targeted species in North America.
关键词：amphibians;conservation;crustaceans;environmental DNA;fish;mollusks;primers;reptiles