文章基本信息

标题：A Newly Designed Experimental System for Exposure of Mammalian Cells to Extremely Low Frequency Magnetic Fields.
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作者：JUNJI MIYAKOSHI ; SHUJI OHTSU ; JUNKO TATSUMI-MIYAJIMA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：1994
卷号：35
期号：1
页码：26-34
DOI：10.1269/jrr.35.26
摘要：To examine the biological effects of extremely low frequency magnetic field (ELFMF), we have designed and manufactured a new equipment for long-term and high-density exposure of cells to ELFMF. The ELFMF exposure system consists of a generator of magnets with a bult-in CO 2 incubator, an alternating current (AC) power supply, a gas compressor and a thermocontroller for the incubator, and a cooling unit for the magnets. The CO 2 incubator made of acrylic resin is inserted into the inner-space of the silicon steel strip-cores. In this system, the temperature of the incubator is maintained at 37±0.5°C. The maximum magnetic flux density on the exposure area of the incubator is 500 mT (T; tesla) at a current of 556 Arms (rms ; root mean square) at 50 Hz. The long-term (up to 120 hr) exposure of 400 mT ELFMF did not affect the growth of both HL60RG and CCRF-CEM cells originated from human leukemia. The post-X-irradiation exposure of 400 mT ELFMF for 2 hr also did not affect the radiation sensitivity of GM0637 and TAT2SF cells originated from a normal human and an ataxia telangiectasia patient.
关键词：Magnetic field exposure system; Extremely low frequency magnetic field; Cell growth; Radiation sensitivity