文章基本信息

标题：Extension of In vitro Life-span of .GAMMA.-irradiated Human Embryo Cells Accompanied by Chromosome Instability.
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作者：MASAO SUZUKI ; ZHI YANG ; KAZUSHIRO NAKANO 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：1998
卷号：39
期号：3
页码：203-213
DOI：10.1269/jrr.39.203
摘要：We studied the effect of repeated irradiation with a low dose rate (about 0.0012 cGy per min) of 60 Co γ-rays on the in vitro life-span of human embryo (HE) cells. HE cells were cultured in an incubator that was set in a 60 Co γ-ray-irradiation room, and the irradiation was repeated throughout the life-span of the HE cells (for about 150 to 160 days) on every day base. During this period, the cells accumulated 106 to 123 cGy. The life-span of the irradiated cells prolonged 1.14 to 1.35 times when compared to that of non-irradiated cells. The incidence of cells with chromosome bridge and micronuclei significantly increased in the irradiated cells. Although the number of chromosomes gradually changed with repetition of culture in both non-irradiated and irradiated cells, the frequencies of aneuploid cells in irradiated cells were about two times higher than that in non-irradiated cells. These results indicate that repeated irradiation with a low dose of γ-rays produces chromosome instability, and it may be a cause of numerical chromosome abnormalities and life-span extension of irradiated cells.
关键词：Low dose γ-rays; Life-span extension; Chromosome bridge; Chromosome instability; Human embryo cells