文章基本信息

标题：Delayed Induction of Telomere Instability in Normal Human Fibroblast Cells by Ionizing Radiation
其他标题：Delayed Induction of Telomere Instability in Normal Human Fibroblast Cells by Ionizing Radiation
本地全文：下载
作者：Mitsuaki OJIMA ; Hiroki HAMANO ; Masatoshi SUZUKI 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2004
卷号：45
期号：1
页码：105-110
DOI：10.1269/jrr.45.105
摘要：We examined the delayed induction of telomere instability in hTERT-immortalized normal human fibroblast (BJ1-hTERT) cells exposed to X-rays. BJ1-hTERT cells were irradiated with 2 Gy of X-rays, and chromosome aberrations were analyzed 24 hours after irradiation and in the surviving cells 14 days after X-ray exposure. We found that the X-ray-surviving cells showed an increased frequency of chromatid gaps and breaks and chromosome fragments compared to the control cells. Furthermore, centromere- and telomere-FISH revealed that the frequency of telomere loss and duplication significantly increased in surviving cells compared to the control level. Because no induction of telomere abnormality was observed in cells 24 hours after irradiation, X-irradiation might not affect telomeres directly, but it specifically induces delayed telomere instability in normal human fibroblast cells.
关键词：Ionizing radiation; Genomic instability; Normal human cells; Telomere