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标题：Retrotransposition of Limited Deletion Type of Intracisternal A-particle Elements in the Myeloid Leukemia Clls of C3H/He Mice
其他标题：Retrotransposition of Limited Deletion Type of Intracisternal A-particle Elements in the Myeloid Leukemia Clls of C3H/He Mice
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作者：Hiroshi ISHIHARA ; Izumi TANAKA ; Hong WAN 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2004
卷号：45
期号：1
页码：25-32
DOI：10.1269/jrr.45.25
摘要：The murine genome has about 1,000 copies of DNA elements for the intracisternal A-particle (IAP) that resembles a retrovirus. We previously reported that the genomic DNA of the cells from radiation-induced acute myeloid leukemia (AML) lines derived from C3H/He inbred mice was frequently rearranged by the integration of the IAP element. In this study, 8 IAP elements from the characteristic integration sites in 6 cell lines of radiation-induced AML from different mice were characterized and compared in structure with 114 IAP elements isolated from the normal C3H/He genome. One of the 8 elements was a full-length type I IAP, and 7 were of type-IΔ1 with a common deletion site. Although the type IΔ1 form is a minor population accounting for about 6% of total genomic IAP elements, it is predominantly retrotransposed in the AML cells from different C3H/He mice. This indicates that limited populations of the IAP elements contribute to the unique retrotransposition in AML cells.
关键词：Genomic IAP element; Physical map of IAP element; Full-length IAP element; Integration; Target duplication