文章基本信息

标题：Distinct Modes of Cell Death by Ionizing Radiation Observed in Two Lines of Feline T-Lymphocytes
其他标题：Distinct Modes of Cell Death by Ionizing Radiation Observed in Two Lines of Feline T-Lymphocytes
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作者：Takehiko KAKIZAKI ; Nobuyuki HAMADA ; Seiichi WADA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2006
卷号：47
期号：3/4
页码：237-243
DOI：10.1269/jrr.0618
摘要：We have examined in vitro radiosensitivities and radioresponses to 60 Co γ-rays irradiation in feline T-lymphocyte cell lines, FeT-J and FL-4. There seemed to be no significant difference in clonogenic survival between the two lines. The mean lethal dose for both was both 1.9 Gy, and surviving fraction at 2 Gy was 0.30 and 0.48 for FeT-J and FL-4 cells, respectively. However, TUNEL assay indicated much higher degrees of apoptosis induction in FeT-J cells (>40%) than in FL-4 cells (<10%) at 4 days after 15 Gy irradiation. Microscopic examination revealed a larger population of multi-nucleate cells in FL-4 cells (60.3%) than in FeT-J cells (16.0%) at 4 days after 15 Gy irradiation, suggesting that a larger ratio of mitotic catastrophe occurred in FL-4 cells. These results suggest that FeT-J is more likely to be induced into apoptosis and FL-4 is more likely to fall into mitotic catastrophe, and eventually necrosis; both of them showed a similar surviving fraction against γ-rays. The results also indicate that FL-4 cells follow a process other than apoptosis to cell death, suggesting the presence of a regulatory mechanism that may control the relationship between mitotic catastrophe and apoptosis in feline T-lymphocytes.
关键词：Feline T-lymphocytes;Apoptosis;Mitotic catastrophe;Clonogenic survival;Ionizing radiation