文章基本信息

标题：Exposure to Strong Magnetic Fields at Power Frequency Potentiates X-ray-induced DNA Strand Breaks
其他标题：Exposure to Strong Magnetic Fields at Power Frequency Potentiates X-ray-induced DNA Strand Breaks
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作者：JUNJI MIYAKOSHI ; MASAMI YOSHIDA ; KEIKO SHIBUYA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2000
卷号：41
期号：3
页码：293-302
DOI：10.1269/jrr.41.293
摘要：We examined the effect of an extremely low-frequency magnetic field (ELFMF) at 5, 50 and 400 mT on DNA strand breaks in human glioma MO54 cells. A DNA damage analysis was performed using the method of alkaline comet assay. The cells were exposed to X-rays alone (5 Gy), ELFMF alone, or X-rays followed by ELFMF at 4°C or on ice. No significant difference in the tail moment was observed between control and ELFMF exposures up to 400 mT. X-ray irradiation increased DNA strand breaks. When cells were exposed to X-rays followed by ELFMF at 50 and 400 mT, the tail moment increased significantly compared with that for X-rays alone. When the exposure of cells was performed at 37°C, no significant change was observed between X-rays alone and X-rays plus 400 mT. We previously observed that exposure to 400 mT ELFMF for 2 h increased X-ray-induced mutations (Miyakoshi et al, Mutat. Res., 349: 109-114, 1996). Additionally, an increase in the mutation by exposure to the ELFMF was observed in cells during DNA-synthesizing phase (Miyakoshi et al., Int. J. Radiat. Biol., 71: 75-79, 1997). From these results, it appears that exposure to the high density ELFMF at more than 50 mT may potentiate X-ray-induced DNA strand breaks.
关键词：Power frequency magnetic field; X-rays; DNA strand breaks; Alkaline comet assay; MO54 cells