文章基本信息

标题：DNA Damage Recognition Proteins Localize along Heavy Ion Induced Tracks in the Cell Nucleus
其他标题：DNA Damage Recognition Proteins Localize along Heavy Ion Induced Tracks in the Cell Nucleus
本地全文：下载
作者：Akihisa TAKAHASHI ; Nobuhiro YAMAKAWA ; Tadaaki KIRITA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2008
卷号：49
期号：6
页码：645-652
DOI：10.1269/jrr.08007
摘要：To identify the repair dynamics involved in high linear energy transfer (LET) radiation-induced DNA damage, phospho-H2AX (γH2AX) foci formation was analyzed after cellular exposure to iron ions (Fe-ions, 500 MeV u -1 , 200 KeV μm -1 ). The foci located at DNA damage sites were visualized using immunocytochemical methods. Since H2AX is phosphorylated at sites of radiation-induced double strand breaks (DSB), γH2AX foci were used to detect or illuminate tracks formed by DSB after exposure to various doses of ionizing radiation. Additional DSB-recognition proteins such as ATM phospho-serine 1981, DNA-PKcs phospho-threonine 2609, NBS1 phospho-serine 343 and CHK2 phospho-threonine 68 all co-localized with γH2AX at high LET radiation induced DSB. In addition, Fe-ion induced foci remained for longer times than X-radiation induced foci. These findings suggest that Fe-ion induced damage is repaired more slowly than X-radiation induced damage, possibly because Fe-ion induced damage or lesions are more complex or extensive. Antibodies for all these phosphorylated DNA DSB recognition proteins appear to be very effective for the detection and localization of DSB.
关键词：Heavy ion particles; Tracks; DSB; γH2AX; DNA damage-recognizing proteins