文章基本信息

标题：Mutation Induction in Cultured Human Cells after Low-dose and Low-dose-rate γ-ray Irradiation: Detection by LOH Analysis
其他标题：Mutation Induction in Cultured Human Cells after Low-dose and Low-dose-rate γ-ray Irradiation: Detection by LOH Analysis
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作者：Yukihiro UMEBAYASHI ; Masamitsu HONMA ; Masao SUZUKI 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2007
卷号：48
期号：1
页码：7-11
DOI：10.1269/jrr.06054
摘要：To study the genetic effects of low-doses and low-dose-rate ionizing radiation (IR), human lymphoblastoid TK6 cells were exposed to 30 mGy of γ-rays at a dose-rate of 1.2 mGy/hr. The frequency of early mutations (EMs) in the thymidine kinase ( TK ) gene locus was determined to be 1.7 × 10 -6 , or 1.9-fold higher than the level seen in unirradated controls. These mutations were analyzed with a loss of heterozygosity (LOH) detection system, a methodology which has been shown to be sensitive to the effects of radiation. Among the 15 EMs observed after IR exposure, 8 were small interstitial-deletion events restricted to the TK gene locus. However, this specific type of event was not found in unirradiated controls. Although these results were observed under the limited conditions, they strongly suggest that the LOH detection system can be used for estimating the genetic effects of a low-dose IR exposure delivered at a low-dose-rate.
关键词：Low-dose and Low-dose-rate γ-ray; LOH analysis; TK6 cell