文章基本信息

标题：Quantitative Detection of Apoptotic Thymocytes in Low-dose X-irradiated Mice by an Anti-single-stranded DNA Antibody
其他标题：Quantitative Detection of Apoptotic Thymocytes in Low-dose X-irradiated Mice by an Anti-single-stranded DNA Antibody
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作者：KAZUKO FUJITA ; YOSHIHIKO KAWARADA ; KUNIHIKO TERADA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2000
卷号：41
期号：2
页码：139-149
DOI：10.1269/jrr.41.139
摘要：The quantitative detection of apoptotic cells in low frequency in the thymus of mice irradiated with X-rays using an anti-single-stranded DNA antibody was explored. The antibody against single-stranded DNA (anti-ssDNA) was obtained with rabbits hyperimmunized with complexes of alkaline-denatured calf thymus DNA. AKR female mice were irradiated with 10 to 100 cGy or 4 Gy X-rays; thereafter, thymus sections were prepared at various times after irradiation. The detection and counting of apoptotic cells in the section were performed after histochemical staining using an anti-ssDNA antibody. The results demonstrate that, although sensitive and quantitative detection of apoptotic cells in irradiated thymus using the anti-ssDNA antibody is possible, the sensitivity is lower compared to that of in situ endlabeling methods, such as TUNEL or ISEL. The antibodies could also be used for rat thymus and spleen. In addition, an increase in positively stained cells by both methods was detected as early as 6 min after the irradiation of mice.
关键词：Antibody; Single-stranded DNA; Detection of apoptosis; Mouse thymus; X-rays