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标题：Growth Cone Collapse and Neurite Retractions: An Approach to Examine X-irradiation Affects on Neuron Cells
其他标题：Growth Cone Collapse and Neurite Retractions: An Approach to Examine X-irradiation Affects on Neuron Cells
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作者：Wael S. AL-JAHDARI ; Yoshiyuki SUZUKI ; Yukari YOSHIDA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2008
卷号：49
期号：5
页码：481-489
DOI：10.1269/jrr.08013
摘要：The growth cone is a structure at the terminal of a neurite that plays an important role in the growth of the neurite. The growth cone collapse assay is considered to be a useful method to quantify the effects of various factors on nerve tissue. Here, we investigated the effect of x-irradiation on growth cones and neurites and also the comparative radiosensitivity of different neurons. Dorsal root ganglia and sympathetic chain ganglion were isolated from day-8 and -16 chick embryos and cultured for 20 h. Neurons were then exposed to x-irradiation and morphological changes were quantitatively evaluated by growth cone collapse assay. Cell viability was examined using TUNEL and WST-1 assays. The results showed that radiation induced growth cone collapse and neurite retraction in a time- and exposure-responsive manner. Growth cone collapse, apoptosis and WST-1 assays showed that no significant difference between the neurons throughout the study period (p ≥ 0.5) after irradiation. Both types of day-8 neurons were more radio-sensitive than day-16 neurons (p ≤ 0.05). The time course of the growth cone collapse was significantly correlated with the apoptotic and cell viability responses at different irradiation doses. Growth cone collapse may represent a useful marker for assaying the effect of x-irradiation on normal cell neurons.
关键词：Growth cone collapse; Neurite retractions; X-rays; DRG; Sympathetic neurons