文章基本信息

标题：Exogenous Expression of Exonuclease Domain-deleted WRN Interferes with the Repair of Radiation-induced DNA Damages
其他标题：Exogenous Expression of Exonuclease Domain-deleted WRN Interferes with the Repair of Radiation-induced DNA Damages
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作者：Genro KASHINO ; Seiji KODAMA ; Keiji SUZUKI 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2005
卷号：46
期号：4
页码：407-414
DOI：10.1269/jrr.46.407
摘要：Werner syndrome (WS) is an autosomal recessive disease characterized by multiple progeroid features. The gene responsible for WS, WRN , is a member of the human RecQ helicase family. WRN is unique among this family, associated with an exonuclease activity. In the present study, we established the human 293-derived cell lines, which expressed exogenously truncated WRN protein, lacking the N-terminal exonuclease domain but having normal helicase activity, and found that they were slightly, but nonetheless significantly, radiosensitive than control cell lines, into which the empty vector had been introduced. The truncated WRN-expressing cells also exhibited increased numbers of micronuclei, chromosome aberrations, and the foci of phosphorylated histone H2AX with X-rays. These results suggested a function of WRN exonuclease activity that is separable from helicase activity and is essential for the repair of radiation-induced DNA damages.
关键词：WRN exonuclease; Radiosensitivity; DNA double-strand breaks; DNA repair; X-rays