文章基本信息

标题：Pretreatment with Rituximab Enhances Radiosensitivity of Non-Hodgkin's Lymphoma Cells
其他标题：Pretreatment with Rituximab Enhances Radiosensitivity of Non-Hodgkin's Lymphoma Cells
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作者：Ira SKVORTSOVA ; Bela-Andre POPPER ; Sergej SKVORTSOV 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2005
卷号：46
期号：2
页码：241-248
DOI：10.1269/jrr.46.241
摘要：The present study examines the effects of ionizing radiation in combination with rituximab (RTX), a chimeric human anti-CD20 monoclonal antibody, on proliferation, cell cycle distribution and apoptosis in B-lymphoma RL and Raji cells. Exposure to ionizing radiation (9 Gy) induced cell growth delay and apoptosis in RL cells, whereas Raji cells showed moderate radio-resistance. The simultaneous exposure of lymphoma cells to ionizing radiation and RTX (10 μg/mL) markedly enhanced apoptosis and cell growth delay in RL and Raji cells. Cooperative antiproliferative and apoptotic effects of RTX and radiation were achieved through the inhibition of c-myc and bcl-XL expression. Furthermore, RTX-modulated expression of cell cycle regulating proteins, such as p53, p21/WAF1, p27/KIP1, contributed to the development of radiation-induced cell killing and growth arrest. Each NHL cell line that underwent apoptosis induced by combination treatment revealed enhanced caspase-3 and poly (ADP-ribose) polymerase (PARP) cleavage as compared to only irradiated cells. These findings show that rituximab synergistically enhances radiation-induced apoptosis and cell growth delay through the expression of proteins involved in the programmed cell death and cell cycle regulation pathways.
关键词：Non-Hodgkin's lymphoma; Radiation; Rituximab; Apoptosis; Cell cycle