文章基本信息

标题：Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells
其他标题：Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells
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作者：MIKINORI KUWABARA ; KENJI TAKAHASHI ; OSAMU INANAMI 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2003
卷号：44
期号：3
页码：203-209
DOI：10.1269/jrr.44.203
摘要：A post-irradiation treatment of the human leukemia cell line MOLT-4 with the anitoxidant Trolox attenuated capase-3 dependent apoptosis. The increase in the p53 expression and SAPK/JNK activation after X irradiation was also inhibited by a Trolox treatment, but the expression of BCL-2 and BAX, which would occur downstream from p53, was not changed. Studies on the effects of the intracellular calcium chelator BAPTA-AM on the induction of apoptosis and the activation of SAPK/JNK and capase-3 proved that the chelation of calcium merely delayed the onset of radiation-induced apoptosis and the activation of SAPK/JNK and caspase-3. When the effects of the protein synthesis inhibitor cycloheximde on the apoptotic signaling pathways, including the activation of caspase family proteins and SAPK/JNK, were investigated, the expression of death receptor Fas through SAPK/JNK activation was found to be required for radiation-induced apoptosis. Finally, the relationship between the amounts of DNA dsb and induction of apoptosis was examined by irradiating BrdU-incorporated cells. An increase in DNA dsb caused by BrdU was found, but the induction of apoptosis was not enhanced. From these data, we could get no positive evidence for DNA as a target of X-rays and p53 as an indispensable factor to induced apoptosis in X-irradiated MOLT-4 cells.
关键词：MOLT-4; Apoptosis; Fas; SAPK·JNK; X irradiation