文章基本信息

标题：Detection of DNA Damage in Individual Cells Induced by Heavy-ion Irradiation with an Non-denaturing Comet Assay
其他标题：Detection of DNA Damage in Individual Cells Induced by Heavy-ion Irradiation with an Non-denaturing Comet Assay
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作者：SEIICHI WADA ; YASUHIKO KOBAYASHI ; TOMOO FUNAYAMA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2002
卷号：43
期号：S
页码：153-156
DOI：10.1269/jrr.43.S153
摘要：Investigating the biological effects of high-LET heavy-ion irradiation at low fluence is important to evaluate the risk of radiation in space. It is especially necessary to detect radiation damage induced by a precise number of heavy ions in individual cells. We thus compared the number of ions traversing a cell and the DNA damage produced by ion hits. We applied a comet assay to measure the DNA damage in individual cells. Cells attached on ion-track detector (CR-39) were irradiated with 17.3 MeV/u 12 C, 15.7 MeV/u, 10.4 MeV/u 20 Ne ion and 7.2 MeV/u 40 Ar beams at TIARA, JAERI-Takasaki. After irradiation, CR-39 was covered with 1% agarose. The agarose was allowed to solidify on a glass slide, and then the electrophoresis was performed. Afterward, the CR-39 was taken off the glass slide. The agarose gel on the CR-39 was stained with ethidium bromide and the opposite side of the CR-39 was etched with a KOH-ethanol solution at 37°C. We observed that heavy ions with higher LET values induced heavier DNA damage, even with the same number of ion hits within the irradiated cells. The result indicated that the amount of DNA damage induced by one particle depended on the LET value of the heavy ions.
关键词：Comet assay; DNA damage; High LET; Individual cell; Ion beam