文章基本信息

标题：Increased Base Change Mutations at G:C Pairs in Escherichia coli Deficient in Endonuclease III and VIII
其他标题：Increased Base Change Mutations at G:C Pairs in Escherichia coli Deficient in Endonuclease III and VIII
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作者：KEIZO TANO ; YOICHI IWAMATSU ; SHINJI YASUHIRA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：2001
卷号：42
期号：4
页码：409-409
DOI：10.1269/jrr.42.409
摘要：Various types of mutation induced by oxidative DNA damage, induced by hydrogen peroxide and riboflavin photosensitization, were determined in Escherichia coli ( E. coli ) mutants deficient in endonuclease III (endo III) and endonuclease VIII (endo VIII). The majority of hydrogen peroxide-induced and spontaneous mutations consisted of G:C to A:T and to T:A base changes, shown on the mutation assay system by a reversion at a specific site of the lacZ gene. Base changes were also localized at G:C pairs in the mutation of the supF gene, induced by riboflavin photosensitization, which specifically yields 7,8-dihydro-8-oxoguanine (8-oxoG). G:C to T:A and to C:G transversions dominated in both mutants. These results suggest that endo III and endo VIII are involved in the repair of oxidative lesions of guanine.
关键词：Endonuclease III;Endonuclease VIII;Oxidative damages;Escherichia coli