文章基本信息

标题：Effect of .GAMMA.-Irradiation on the Uptake and Digestion of 125I-labeled Bovine Serum Albumin by Rat Visceral Yolk Sac Cultured in vitro.
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作者：SENTARO TAKAHASHI ; CHIHIRO KOSHIMOTO ; YOSHIHISA KUBOTA 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：1993
卷号：34
期号：2
页码：171-176
DOI：10.1269/jrr.34.171
摘要：The effect of γ-irradiation on a major nutritional function of the yolk sac (the uptake and digestion of macromolecular materials) was studied in rat visceral yolk sacs cultured in vitro being used 125 I-labeled bovine serum albumin (RISA) as the tracer protein. The uptake of RISA (per g wet weight) by rat yolk sacs irradiated with doses of 5-80 Gy was essentially the same as that in the unirradiated control yolk sacs. There were no significant differences in yolk sac uptake of RISA with respect to the radiation doses or to culture period up to 18 hours after irradiation. External γ-irradiation with 10-80 Gy doses also had no effect on the extracellular release of 125 I from yolk sacs which had been taken it up as RISA. The ratios of the activity in ultrafiltrates of the medium to the total activity in the medium were slightly higher at doses of 40 and 80 Gy.
关键词：γ-irradiation; bovine serum albumin; yolk sac; endocytic uptake; intracellular digestion