文章基本信息

标题：Determination of total free amino acids in Sipunculus nudus by UV spectrophotometry
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作者：Hongli Ye ; Youqiong Cai ; Longfei Zhang 等
期刊名称：E3S Web of Conferences
印刷版ISSN：2267-1242
电子版ISSN：2267-1242
出版年度：2020
卷号：189
页码：1-6
DOI：10.1051/e3sconf/202018902013
出版社：EDP Sciences
摘要：Here we developed a new, facile method to detect free amino acids (TFAA) in Sipunculus nudus by UV spectrophotometry. The pretreatment conditions of the approach were optimized, such as chromogenic reaction temperature and time, the measurement time after chromogenic reaction, the extraction agent and its concentration, the extraction time. The established method showed a good linearity in the TFAA range 0.0 ~ 40.0 cg/mL (R 2 = 0.9958) with the limit of detection (LOD) 1.96 g /mL and the precision of 6.14 % (relative standard deviation, RSD). The addition standard recoveries were 95.1 % ~108.6 %. Finally, the method was successfully applied in detection of TFAA in Sipunculus nudus and other aquatic products. The results showed that the content of TFAA in Sipunculus nudus was higher than that in the other aquatic products collected in our paper, and as high as 138.14 g/kg.