文章基本信息

标题：Induction of callus in Cassia mimosoides
本地全文：下载
作者：Yali Yang ; Peiting He ; Jiayi Zhang 等
期刊名称：E3S Web of Conferences
印刷版ISSN：2267-1242
电子版ISSN：2267-1242
出版年度：2020
卷号：189
页码：1-5
DOI：10.1051/e3sconf/202018902017
出版社：EDP Sciences
摘要：In this study, the young leaves of Cassia mimosoides were used as explants. On the basis of 1/2 MS 2.0 mg/L 6-BA 0.5 mg/L NAA medium, we observed the effect of adding different factors on the callus induction of C. mimosoides . The results shown that after 20 days of callus induction, it was found that the addition of 150 mg/L vitamin C (VC) did not affect the induction efficiency, but the growth of callus became slower; and when the concentration was higher than 150 mg/L, the formation of callus would be inhibited. The induction of callus would be restrained while adding different concentrations of Cu 2 into mediums. Moreover, glutamine (Gln) had little effect on the induction of callus. Furthermore, the addition of hydrolyzed casein (CH) would not affect the formation of callus, but the high concentration of CH could affect the growth status of callus. The induction efficiency of callus was severely inhibited by exogenous addition of silver nitrate (AgNO3), and the growth status of callus was poor, and the phenomenons of early rooting had happened at the same time. The results of this study provided a theoretical basis for the subsequent optimization of bud proliferation and rooting in C. mimosoides .