文章基本信息

标题：Increased Production of Coenzyme $Q_{10}$ from Genetic Engineered Rhodobacter sphaeroides Overexpressing UbiG
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作者：Zhiping Zhao ; Ziyi Ye ; Xinning Xu 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2020
卷号：18
期号：2
页码：22-26
DOI：10.19026/ajfst.18.6051
出版社：MAXWELL Science Publication
摘要：The aim of this study was to increase the Coenzyme $Q_{10}$ $(CoQ_{10})$ yield from Rhodobacter sphaeroides via genetic engineering pathway. $CoQ_{10}$ plays important roles in many biological processes and has been proven to be effective in the treatment of many diseases. In the present study, the ubiG gene located in $CoQ_{10}$ biosynthesis pathway was effectively overexpressed in Rb. Sphaeroides to increase $CoQ_{10}$ production. The growth of host cells was slightly influenced by overexpressing ubiG . The crude $CoQ_{10}$ production was enhanced by 58.31% compared to that from the control. The ubiG mRNA level was significantly increased compared to the wild type harboring empty vector as measured by qRT-PCR. Moreover, the crude $CoQ_{10}$ exhibited strong anti-oxidant activity as measured in vivo by zone of inhibition assay.
关键词：Anti-oxidation; $CoQ_{10}$; genetic engineering; hydroxyl radical scavenging activity; Rb. Sphaeroides; ubiG