文章基本信息

标题：Transcriptome analysis reveals that the multiple metabolic pathways were related to gluten polymerization in different quality wheats (Triticum aestivum L.)
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作者：Qi Wang ; Feng Jia ; Xia Zhang 等
期刊名称：Food Science & Nutrition
电子版ISSN：2048-7177
出版年度：2020
卷号：8
期号：8
页码：4573-4583
DOI：10.1002/fsn3.1769
出版社：John Wiley & Sons, Ltd.
摘要：The rapid development of transcriptome sequencing technology has contributed to the discovery of numerous genes in plant; however, the role of gene expression in postharvest wheat remains largely unexplored. In this study, differentially expressed genes (DEGs) were identified by RNA‐seq in different quality wheats. The 102.6 Gb clean reads had been yielded from the nine RNA‐seq libraries. Typically, there were 1791 upregulated and 2,677 downregulated DEGs, respectively, in strong‐gluten wheat compared with weak‐gluten wheat. Specifically, a total of 4,468 DEGs were classified into 286 Gene Ontology (GO) terms and 131 Kyoto Encyclopedia of Genes and Genomes terms (KEGG). Moreover, the storage protein components, starch and sucrose metabolism, and plant hormone signal transduction‐related genes were discovered, which had involved 109 DEGs. The wet gluten proteins content was 35.24% and 17.36%, and the glutenin macropolymer content was 6.38% and 5.01% between the strong‐ and weak‐gluten wheat, respectively. The POD activities of the different quality wheats were 6,571.14, 5,341.24, and 4,851.48 U/g/min, respectively. The significant difference of starch and sucrose metabolism, hormone, POD, and CAT enzyme along with the higher ATPase activity might potentially affect gluten polymerization, which might thereby result in the different qualities of wheats.
关键词：gluten polymerization;RNA sequence;transcriptome;wheat