文章基本信息

标题：Evaluation of antioxidant and anticancer activities of naphthoquinones‐enriched ethanol extracts from the roots of Onosma hookeri Clarke. var. longiforum Duthie
本地全文：下载
作者：Qiang Wu ; Aga Er‐bu ; Xiaoxia Liang 等
期刊名称：Food Science & Nutrition
电子版ISSN：2048-7177
出版年度：2020
卷号：8
期号：8
页码：4320-4329
DOI：10.1002/fsn3.1729
出版社：John Wiley & Sons, Ltd.
摘要：In this study, the optimal naphthoquinones‐enriched ethanol extract from the roots of Onosma hookeri Clarke. var. longiforum Duthie ( OHC ‐LD) was obtained under an optimal condition (69% ethanol, material to solution ratio of 27:1 at 60℃ for 59 min) by the ultrasound‐assisted extraction, according to four‐variable three‐level Box–Behnken design‐response surface methodology. The experimental yield of ethanol extract was 42.08 ± 0.65%, and the contents of naphthoquinones reached to 1.07 ± 0.004%. The optimal extract exhibited similar scavenging activity against ABTS (2,2'‐azino‐bis‐3‐ethylbenzthiazoline‐6‐sulfonic acid) radical as BHT(butylated hydroxytoluene) at 1,250 µg/ml, and better DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) scavenging activity than BHT at 250 µg/ml. However, the optimal ethanol extract was not sensitive to MCF‐7 cell line ( IC50 of 321.849 µg/ml). The results revealed the naphthoquinones‐enriched ethanol extract from the roots of OHC ‐LD had could be used as a potential natural antioxidant.
关键词：anticancer;antioxidant;naphthoquinones‐enriched ethanol extract;Onosma hookeri Clarke. var. longiforum Duthie