文章基本信息

标题：Control of pH by CO2 Pressurization for Enzymatic Saccharification of Ca(OH)2-Pretreated Rice Straw in the Presence of CaCO3
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作者：Masakazu Ike ; Ken Tokuyasu
期刊名称：Journal of Applied Glycoscience
印刷版ISSN：1344-7882
电子版ISSN：1880-7291
出版年度：2020
卷号：67
期号：2
页码：59-62
DOI：10.5458/jag.jag.JAG-2019_0019
出版社：The Japanese Society of Applied Glycoscience
摘要：The aim of this study was to investigate the effect of pH control by CO2 pressurization on the enzymatic hydrolysis of herbaceous feedstock in the calcium capturing by carbonation (CaCCO) process for fermentable sugar production. The pH of the slurry of 5 % (w/w) Ca(OH)2 -pretreated/CO2 -neutralized rice straw could be controlled between 5.70 and 6.38 at 50 °C by changing the CO2 partial pressure ( p CO2 ) from 0.1 to 1.0 MPa. A mixture of fungal enzyme preparations, namely, Trichoderma reesei cellulases/hemicellulases and Aspergillus niger β-glucosidase, indicated that pH 5.5–6.0 is optimal for solubilizing sugars from Ca(OH)2 -pretreated rice straw. Enzymatic saccharification of pretreated rice straw under various p CO2 conditions revealed that the highest soluble sugar yields were obtained at p CO2 0.4 MPa and over, which is consistent with the expected pH at the p CO2 without enzymes and demonstrates the effectiveness of pH control by CO2 pressurization.
关键词：calcium capturing by carbonation (CaCCO) process;CO2-pressurized enzymatic saccharification;fungal cellulase;fungal β-glucosidase