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  • 标题:Potent Antibacterial Activity of Synthetic Peptides Designed from Salusin-β and HIV-1 Tat(49–57)
  • 本地全文:下载
  • 作者:Masahiro Kimura ; Kumiko Kosuge ; Yui Ko
  • 期刊名称:Chemical and Pharmaceutical Bulletin
  • 印刷版ISSN:0009-2363
  • 电子版ISSN:1347-5223
  • 出版年度:2020
  • 卷号:68
  • 期号:8
  • 页码:810-813
  • DOI:10.1248/cpb.c20-00209
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Salusin-β is an endogenous bioactive peptide that was identified in a human full-length enriched cDNA library using bioinformatics analyses. In our previous study, we found that synthetic salusin-β exhibits antibacterial activity against only Gram-positive microorganisms such as Staphylococcus aureus NBRC 12732. Salusin-β has an ability to depolarize the cytoplasmic membrane of this bacterium, and this phenomenon may be linked to the antibacterial activity of this peptide. A cell-penetrating peptide (CPP), human immunodeficiency virus (HIV)-1 transactivator of transcription (Tat) (49–57) is a short cationic peptide that can traverse cell membranes. In this report, synthetic peptide conjugates of salusin-β and HIV-1 Tat(49–57) showed potent antibacterial activities against both Gram-positive Staphylococcus aureus NBRC 12732 and Gram-negative Escherichia coli NBRC 12734. The synthetic peptides also depolarized the cytoplasmic membrane of Escherichia coli NBRC 12734 as well as Staphylococcus aureus NBRC 12732. These results suggested that HIV-1 Tat(49–57) is a protein transduction domain or CPP that changes the interaction mode between salusin-β and the cell membrane of Escherichia coli NBRC 12734. By binding to HIV-1 Tat(49–57), salusin-β showed a broad antibacterial spectrum regardless of whether the target was a Gram-positive or Gram-negative bacterium.
  • 关键词:salusin-β;antibiotic activity;conjugate;human immunodeficiency virus-1 transactivator of transcription (49–57);antibacterial action mechanism
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