文章基本信息

标题：Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols
本地全文：下载
作者：Chia-Chen Chang ; Tsz-Lian Hsu ; Chie-Pein Chen 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：9
页码：1-10
DOI：10.3390/bios10090113
出版社：MDPI Publishing
摘要：A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.
关键词：colorimetric assay; catalytic gold nanoparticle; iodine; peroxidase-like activity colorimetric assay ; catalytic gold nanoparticle ; iodine ; peroxidase-like activity