文章基本信息

标题：Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences
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作者：Long Chen ; Xingye Chen ; Xusan Yang 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2020
卷号：18
页码：2209-2216
DOI：10.1016/j.csbj.2020.06.038
出版社：Computational and Structural Biotechnology Journal
摘要：Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, suffering from optical diffraction limit, conventional FPM has low orientation resolution and observation accuracy, as the polarization information is averaged by multiple fluorescent molecules within a diffraction-limited volume. Recently, novel super-resolution FPMs have been developed to break the diffraction barrier. In this review, we will introduce the recent progress to achieve sub-diffraction determination of dipole orientation. Biological applications, based on polarization analysis of fluorescence dipole, are also summarized, with focus on chromophore-target molecule interaction and molecular organization.
关键词：Fluorescence polarization microscopy ; Dipole orientation ; Super-resolution ; Biomolecule interactions ; Molecular organization