文章基本信息

标题：Antibacterial Activity of The Secondary Metabolites Produced by Endophytic Fungi Isolated from Acanthus ilicifolius Plant
本地全文：下载
作者：Gusti Ayu Widayanti ; Hary Widjajanti ; Salni 等
期刊名称：IJEMS (Indonesian Journal of Environmental Management and Sustainability)
印刷版ISSN：2598-6260
电子版ISSN：2598-6279
出版年度：2019
卷号：3
期号：4
页码：107-116
DOI：10.26554/ijems.2019.3.4.107-116
语种：English
出版社：ARTS Publishing Corp.
摘要：Endophytic fungi can produce secondary metabolites that are same as the host.Twelve endophytic fungi were isolated from the Acanthus ilicifolius plant.Two of them have antibacterial activity,DJS1 and DJS2.Fungi can produce secondary metabolites that have the potential as antibacterial compounds.This study aims to isolate antibacterial compounds using column chromatography methods and test the antibacterial activity using diffusion methods to use paper discs.Isolation of secondary metabolites produces 27 eluates.From TLC,eluate DJS11 and eluate DJS22 contains phenol compound,eluate DJS24 contains terpenoids compound,and eluate DJS25 contain alkaloids copound,each component of the active compound are tested for sensitivity to bacterias Escherichia coli,Staphylococcus aureus,Shigella dysenteriae,and Salmonella typhi.MIC value of Escherichia coli on DJS11 eluate is 500 ppm,DJS22 eluate is 250 ppm,DJS24 eluate is 250 ppm,and DJS25 eluate is 250 ppm.MIC value of Staphylococcus aureus on DJS11 eluate is 500 ppm,DJS22 is 1000 ppm eluate,DJS24 eluate is 125 ppm,and DJS25 eluate is 500 ppm.MIC value of Shigella dysenteriae on DJS11 eluate is 500 ppm,DJS22 eluate is 1000 ppm,DJS24 eluate is 250 ppm,and DJS25 eluate is 500 ppm.MIC value of Salmonella typhi on DJS11 eluate is 125 ppm,DJS22 eluate is 125 ppm,DJS24 eluate is 125 ppm,and DJS25 eluate is 125 ppm.
关键词：Acanthus ilicifolius;MIC;Antibacterial compound