期刊名称:IJEMS (Indonesian Journal of Environmental Management and Sustainability)
印刷版ISSN:2598-6260
电子版ISSN:2598-6279
出版年度:2019
卷号:3
期号:4
页码:100-106
DOI:10.26554/ijems.2019.3.4.100-106
语种:English
出版社:ARTS Publishing Corp.
摘要:Endophytic fungi can produce secondary metabolites.The purpose of this study is to identify and examine the genetic relationship of endophytic fungi isolates with ITS markers.The endophytic fungi isolate DKJ1,DKJ3a,DKJ3c,and DKJ4 were successfully isolated from the Cardia plant (Bellucia pentamera Naudin) indicated by Aspergillus niger group,Aspergillus fumigatus group and Penicillium sp.The results of identification and analysis of DNA the sequencing of endophytic fungi DKJ1,DKJ3c,DKJ4,and DKJ3a with the primary pair of ITS shows that the phylogenetic tree is different from the species obtained isolate DKJ1 has a similarity index value of 1,000 with Aspergillus piperis CBS 112811 species and 1,000 similarity index values with Aspergillus luchuensis KACC 46772 species,DKJ3c has a similarity index value of 1,000 with the species Aspergillus flavus var flavus strain ATCC 16833,DKJ4 has a similarity index value of 1,000 with the Penicillium oxalicum CBS 219.30 species and has a similarity index value of 1,000 with the Penicillium oxalicum strain NRRL 787 species and isolate DKJ3a has a similarity index value of 1,000 with the Penicillium roflsii strain NRRL 1078 species.But there are similarities based on Cluster A (Aspergillus Group) and Cluster B (Penicillium Group) on phylogenetic trees.