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标题：Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication
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作者：PATRICIA REGINA SANTOS ; ANELISE S. NAZARI FORMAGIO ; BENEDITO PRADO DIAS FILHO 等
期刊名称：Acta Pharmaceutica
电子版ISSN：1846-9558
出版年度：2015
卷号：65
期号：1
页码：75-81
DOI：10.1515/acph-2015-0004
语种：English
出版社：Sciendo
摘要：The effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU).Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell.The compound was able to prevent CPE and PFU formation when the cells were pretreated with PMC for 24 h prior to PV infection.In addition,when the alkaloid was continuously maintained in the infected cultures,the spread of PV to adjacent cells was impaired.The pre-exposure and post-exposure prophylactic applications are possible situations in which an anti-PV drug might be used.Future studies are needed to elucidate the PMC mode of action and verify the feasibility of PMC use in vivo.No antipicornavirus agent is currently approved for clinical use.
关键词：1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3;4-b]indole-3-carbohydrazide;b-carboline;poliovirus;antiviral activity