文章基本信息

标题：Optimized and validated flow-injection spectrophotometric analysis of topiramate,piracetam and levetiracetam in pharmaceutical formulations
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作者：GHADA M. HADAD ; RANDA A. ABDEL-SALAM ; SAMY EMARA 等
期刊名称：Acta Pharmaceutica
电子版ISSN：1846-9558
出版年度：2011
卷号：61
期号：4
页码：377-389
DOI：10.2478/v10007-011-0038-y
语种：English
出版社：Sciendo
摘要：Application of a sensitive and rapid flow injection analysis (FIA) method for determination of topiramate,piracetam,and levetiracetam in pharmaceutical formulations has been investigated.The method is based on the reaction with ortho-phtalaldehyde and 2-mercaptoethanol in a basic buffer and measurement of absorbance at 295 nm under flow conditions.Variables affecting the determination such as sample injection volume,pH,ionic strength,reagent concentrations,flow rate of reagent and other FIA parameters were optimized to produce the most sensitive and reproducible results using a quarter-fraction factorial design,for five factors at two levels.Also,the method has been optimized and fully validated in terms of linearity and range,limit of detection and quantitation,precision,selectivity and accuracy.The method was successfully applied to the analysis of pharmaceutical preparations.
关键词：topiramate;piracetam;levetiracetam;flow-injection analysis