文章基本信息

标题：Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent
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作者：Risa Fitriandini ; Risa Fitriandini ; Sri Budiarti 等
期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2017
卷号：9
期号：3
页码：600-607
DOI：10.15294/biosaintifika.v9i3.10323
语种：English
出版社：Universitas Negeri Semarang
摘要：Rhododendron has been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria have not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assessed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in the ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escherichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twentythree of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e., RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 o C. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/ mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contributes to the understanding of their diversity and potency as an antibacterial agent.
关键词：Actinobacteria; Antibacterial activity; Endophyte; Rhododendron spp